2/29/2024 0 Comments Southern blight tomato![]() ![]() Colonies with similar morphology were chosen for re-isolation, and further phylogenetic analysis verified the presence of the three isolates. For ensurimg bacterial colonization inside the plants, tomato seeds were treated with bacterial suspension and allowed to grow in water agar. In addition, the percent disease incidence (DI) and percent disease index (PDI) of southern blight disease decreased significantly and yield of the tomato improved significantly. Soil amendments and seed treatment with the three isolates considerably increased the growth characteristics of tomato plants, including germination percentage, root length, shoot length, fresh weight, and dry weight. Phylogenetic analysis of the isolates PLPTw and PLPTu identified them as Burkholderia contaminans and PLPKb as Bacillus velezensis. However, only PLPTw and PLPKb were positive for phosphate solubilization, with a phosphate solubilization efficiency of 4.05% (PLPTw) and 3.16% (PLPKb) respectively. All the three isolates were positive in indole-3-acetic acid and biofilm production. rolfsii which was recorded as 91.11%, while the extracellular metabolite produced by the three isoates inhibited 100% growth of S. The isolate PLPTu showed impressive mycelial growth inhibition of S. ![]() Three isolates namely PLPKb, PLPTw, PLPTu showed a varied level of antagonism against S. In this study, endophytic bacteria isolated from three medicinal plants, namely Cantella asiatica, Ocimum sanctum, Solanum sisymbriifolium were evaluated for their plant growth-promoting (PGP) activity and antagonistic potential against tomato southern blight pathogen Sclerotium rolfsii.
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